Biochemical Techniques I 

Bio 3037 

Thur:10:00-1:00

 Text Book: Experimental Biochemistry: theory and exercises in fundamental methods. 3rd edition.  By Robert Switzer & Liam Garrity ; Freeman Publisher

 

Date

Topic

Experiment (Page)

8/31 Thur

Introduction & Experimental Data

1-10

9/7 Thur

Problem solving, Use of pH meter, Reagent preparation

 

9/14 Thur

Photometry

15-22

9/21 Thur

Chromatograhy

25-43

9/28 Thur

Exam I (20%)

 

10/5 Thur

Quantification of Protein

               93-94

10/12 Thur

No class (Dr. Cheung attends a conference)

 

10/19 Thur

Reagent Preparation:  SDS-PAGE  continue

               61-77

10/26 Thur

SDS-PAGE Electrophoresis

 

11/2 Thur

Exam II (20%)

            

11/9 Thur  

Reagent Preparation: DNA

 

11/16 Thur  

Isolation of DNA                333-336  

11/30 Thur

Characterization of DNA, DNA enzyme digestion

             318-326

12/7 Thur

Agarose gel Electrophoresis

72-74

12/14 Thur

Exam III (20%)

 

 Lab Reports, Assignments & Quiz: 35%

Attendance & Performance: 5%

* Graduate students require finishing an extra project

**No make-up exams will be given except emergency, documents must be provided under those circumstances

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